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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- PA5-21549 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCAT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: BCAT2-transfected 293T. Predicted reactivity: Mouse (85%), Rat (83%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.19 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression.
Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failure.
Cardiac branched-chain amino acid oxidation is reduced during insulin resistance in the heart.
Ericksen RE, Lim SL, McDonnell E, Shuen WH, Vadiveloo M, White PJ, Ding Z, Kwok R, Lee P, Radda GK, Toh HC, Hirschey MD, Han W
Cell metabolism 2019 May 7;29(5):1151-1165.e6
Cell metabolism 2019 May 7;29(5):1151-1165.e6
Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failure.
Uddin GM, Zhang L, Shah S, Fukushima A, Wagg CS, Gopal K, Al Batran R, Pherwani S, Ho KL, Boisvenue J, Karwi QG, Altamimi T, Wishart DS, Dyck JRB, Ussher JR, Oudit GY, Lopaschuk GD
Cardiovascular diabetology 2019 Jul 5;18(1):86
Cardiovascular diabetology 2019 Jul 5;18(1):86
Cardiac branched-chain amino acid oxidation is reduced during insulin resistance in the heart.
Fillmore N, Wagg CS, Zhang L, Fukushima A, Lopaschuk GD
American journal of physiology. Endocrinology and metabolism 2018 Nov 1;315(5):E1046-E1052
American journal of physiology. Endocrinology and metabolism 2018 Nov 1;315(5):E1046-E1052
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCAT2 using 30 µg of A) A549 (B) H1299 (C) HCT116 and D) MCF-7 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BCAT2 polyclonal antibody (Product # PA5-21549) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCAT2 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BCAT2 polyclonal antibody (Product # PA5-21549) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BCAT2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCAT2 Polyclonal Antibody (Product # PA5-21549) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 (Product # PA5-21549) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 (Product # PA5-21549) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
