PA5-31432

antibody from Invitrogen Antibodies

Targeting: ARF5

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31432

Provider

Invitrogen Antibodies

Product name

ARF5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (85%), Pig (100%), Chicken (98%), Sheep (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.

Rennoll-Bankert KE, Rahman MS, Gillespie JJ, Guillotte ML, Kaur SJ, Lehman SS, Beier-Sexton M, Azad AF
PLoS pathogens 2015 Aug;11(8):e1005115

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 Arf6 is recruited by R . typhi RalF and is required for infection. (A) Ectopically expressed RalF RtFL co-localizes with Arf6 but not Arf5. HeLa cells co-expressing EYFP, EYFP-RalF RtFL or EYFP-RalF RbFL and mRFP-Arf6 (left) or -Arf5 (right) were fixed with 4% para-formaldehyde. Nuclei were stained with DAPI (blue). (Scale bar: 10 mum). (B) RalF RtFL pull-down of Arf6. Lysates from HEK293T cells expressing mRFP-Arf5 or -Arf6 were incubated with HisPur Cobalt resin bound with rHis-RalF RtFL or resin alone. Bound proteins were eluted with imidazole and analyzed by protein immunoblot using antibodies as indicated. (C) Arf6 is recruited during R . typhi entry. HeLa cells expressing mRFP-Arf5 or -Arf6 (red) were infected with partially purified R . typhi (MOI ~100). Ten minutes post infection, cells were fixed and R . typhi detected with anti- R . typhi serum (green). DAPI (blue) is shown in the merged image. Boxed regions are enlarged to show detail. White arrowheads indicate R . typhi . (Scale bar: 5 mum). (D) Arf6 knockdown inhibits R . typhi infection. HeLa cells transfected with negative, Arf6, or Arf5 siRNA were infected with partially purified R . typhi (MOI ~100). At 2 hrs post infection, cells were fixed, plasma membrane stained with Alexa Fluor 594 wheat germ agglutinin, and R . typhi detected with rat anti- R . typhi serum and Alexa Fluor 488 anti-rat antibody. The number of R . typhi per host cell was counted for 100 host cells for three independent experiments.