Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX107002 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107002, RRID:AB_1952102
- Product name
- SUCLG2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Screen for abnormal mitochondrial phenotypes in mouse embryonic stem cells identifies a model for succinyl-CoA ligase deficiency and mtDNA depletion.
Donti TR, Stromberger C, Ge M, Eldin KW, Craigen WJ, Graham BH
Disease models & mechanisms 2014 Feb;7(2):271-80
Disease models & mechanisms 2014 Feb;7(2):271-80
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Supportive validation
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- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse liver 10% SDS PAGE GTX107002 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)10% SDS PAGEGTX107002 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using SUCLG2(GTX107002) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SUCLG2 antibody detects SUCLG2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SUCLG2 protein stained by SUCLG2 antibody (GTX107002) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SUCLG2 antibody detects SUCLG2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. SUCLG2 antibody (GTX107002) dilution: 1:500.