PA5-110450

antibody from Invitrogen Antibodies

Targeting: SURF4 ERV29, FLJ22993, MGC102753

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110450

Provider

Invitrogen Antibodies

Product name

SURF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: ACFGLFGIIA LQTIAYSILW DLKFLMRNLA LGGGLLLLLA ESRSEGKSMF AGVPTMRESS PKQYMQLGGR VLLVLMFMTL LHFDASFFSI VQNIVGTALM I

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.46 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SURF4 in extracts of HepG2 cells. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SURF4 in NIH/3T3 cells. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SURF4 in C6 cells. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SURF4 in paraffin-embedded human liver cancer. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SURF4 in paraffin-embedded rat pancreatic islet. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SURF4 in paraffin-embedded rat pancreatic islet. Samples were incubated with SURF4 Polyclonal antibody (Product # PA5-110450) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.