Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00169436-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00169436-M03, RRID:AB_537307
- Product name
- Mouse Monoclonal SgK071 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. C9orf96 - chromosome 9 open reading frame 96
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SgK071 Antibody (2B5) [H00169436-M03] - Western blot analysis of C9orf96 over-expressed 293 cell line. Lane 2 cotransfected with C9orf96 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00169436-M03. GAPDH(36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SgK071 Antibody (2B5) [H00169436-M03] - Analysis of C9orf96 expression in transfected 293T cell line by C9orf96 monoclonal antibody (M03), clone 2B5. Lane 1: C9orf96 transfected lysatE (75.5 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SgK071 Antibody (2B5) [H00169436-M03] - Detection limit for recombinant GST tagged C9orf96 is approximately 0.03ng/ml as a capture antibody.