F54410

antibody from NSJ Bioreagents

Targeting: RRAGD bA11D8.2.1, DKFZP761H171

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54410

Provider

NSJ Bioreagents

Product name

RagD Antibody / RRAGD

Antibody type

Polyclonal

Description

This highly specific RagD antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the RagD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse cerebellum lysate with RagD antibody. Predicted molecular weight: ~51 kDa and ~30 kDa (multiple isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with RagD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-435 cells with RagD antibody; Blue=isotype control, Green= RagD antibody.