Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Immunohistochemistry [1]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - STJ70095 - Provider product page

 - Provider
 - St John's Laboratory
 - Product name
 - Anti-COP1 antibody (isofom 2)
 - Antibody type
 - Polyclonal
 - Host
 - Goat
 - Conjugate
 - Unconjugated
 - Antigen sequence
 ETLGLSAGPIPGN- Epitope
 - C-term
 - Isotype
 - IgG
 - Vial size
 - 100μg,,
 - Concentration
 - 0.5 mg/ml
 - Storage
 - Store at -20°C, and avoid repeat freeze-thaw cycles.
 
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					Supportive validation
					
									
				
		
				- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - STJ70095 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA
 
							
					Supportive validation
					
									
				
		
		- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - STJ70095 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA