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Western blot
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- References [1]
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- Western blot [2]
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- Product number
- GTX106526 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX106526, RRID:AB_11172792
- Product name
- hnRNP R antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE GTX106526 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and hnRNP R knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- hnRNP R antibody [N1N2], N-term detects hnRNP R protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: hnRNP R protein stained by hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
