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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-28473 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa. Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (81%), Chicken (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP R Polyclonal Antibody (Product # PA5-28473). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa . 7.5% SDS PAGE. hnRNP R Polyclonal Antibody (Product # PA5-28473) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP R Polyclonal Antibody (Product # PA5-28473). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa . 7.5% SDS PAGE. hnRNP R Polyclonal Antibody (Product # PA5-28473) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of hnRNP R was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP R Polyclonal Antibody (Product # PA5 28473) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
