PA1-16788

antibody from Invitrogen Antibodies

Targeting: SCARB1 CD36L1, CLA-1, CLA1, SR-BI, SRB1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16788

Provider

Invitrogen Antibodies

Product name

SR-BI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: antigen standard for SCARB1 (transient overexpression lysate).

Reactivity

Human, Mouse, Rat, Hamster, Porcine, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Insulin increases cholesterol uptake, lipid droplet content, and apolipoprotein B secretion in CaCo-2 cells by upregulating SR-BI via a PI3K, AKT, and mTOR-dependent pathway.

Fuentes M, Santander N, Cortés V
Journal of cellular biochemistry 2019 Feb;120(2):1550-1559

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SR-BI in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Samples were incubated in SR-BI polyclonal antibody (Product # PA1-16788) using a dilution of 1:100 dilution in PBS (0.1% Tween) with 1% BSA followed by anti-rabbit conjugate to Alexa Fluor 488. Primary incubation overnight at 4 °C. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SR-BI in human adrenal cortex. Samples were incubated in SR-BI polyclonal antibody (Product # PA1-16788).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SR-BI in formalin-fixed paraffin-embedded tissue section of mouse liver. Samples were incubated in SR-BI polyclonal antibody (Product # PA1-16788) using a dilution of 1:300 followed by HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SR-BI in formalin-fixed paraffin-embedded tissue section of mouse liver. Samples were incubated in SR-BI polyclonal antibody (Product # PA1-16788) using a dilution of 1:300 followed by HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Huh7 and HepG2 cells using SR-B1 antibody, Product # PA1-16788.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SR-BI in HeLa cells (blue) and a matched isotype control (orange). Samples were incubated in SR-BI polyclonal antibody (Product # PA1-16788) using a dilution of 5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 488.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 Insulin increases intracellular neutral lipid content in CaCo-2 cells by mechanisms that require SR-BI-mediated cholesterol uptake. A, CaCo-2 cells were incubated for 24 h with glucose at low (5 mM) or high (25 mM) concentrations, plus insulin (100 nM) and BLT-1 (1 muM) or vehicle and then subjected to confocal microscopy analysis to visualize SR-BI (red), lipid droplets, (green, BODIPY), and nuclei (blue, H33342), as indicated in Section 2. B, Digital orthogonal ( YZ ) projection reconstruction of confocal images of the cell cultures depicted in A, BODIPY, 4,4-difluoro-4-bora-3a,4a-diaza- s -indacene; BLT-1, block lipid transport-1; SR-BI, scavenger receptor, class B, type I

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

2 Insulin increases SR-BI levels in the apical surface of CaCo-2 cells and requires PI3K, AKT, and mTOR activities. A, CaCo-2 cells were incubated with inhibitors for PI3K (wortmannin), AKT (triciribine), or mTOR (rapamycin) for 1 h and then stimulated with 100 nM insulin plus the different inhibitors for additional 24 h, as indicated in Section 2. Whole cell lysates of CaCo-2 cells were analyzed by Western blot. B, CaCo-2 cells were incubated with triciribine or rapamycin and insulin as indicated in (A) and then their cell surface proteins were biotinylated as indicated in Section 2. Biotinylated cell lysates were precipitated with streptavidin and analyzed by Western blot with antibodies to IR and SR-BI as detailed in Section 2. IR, insulin receptor; mTOR, mammalian target of rapamycin; PI3K, phosphatidylinositol 3-kinase; SR-BI, scavenger receptor, class B, type I