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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP21775PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21775PU-N, RRID:AB_10755853
- Product name
- anti EIF4A2
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 50-100 of human eIF4A2
- Reactivity
- Human, Mouse, Canine, Chicken/Avian, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0,5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using AP21775PU-N at 0.05 µg/ml, 0.05 µg/ml and 0.025 µg/ml, respectively.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody (Cat,.-No.: AP21775PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
