MA5-27681

antibody from Invitrogen Antibodies

Targeting: KCNJ2 IRK1, Kir2.1, LQT7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27681

Provider

Invitrogen Antibodies

Product name

Kir2.1 (KCNJ2) Monoclonal Antibody (S112)

Antibody type

Monoclonal

Antigen

Other

Description

1 µg/mL of MA5-27681 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S112

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KCNJ2 in monkey COS transient cell lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with KCNJ2 monoclonal antibody (Product # MA5-27681) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KCNJ2 in paraffin-embedded mouse back skin. Samples were incubated with KCNJ2 monoclonal antibody (Product # MA5-27681) using a dilution of 1:100 (1 hour at RT) and followed by FITC Goat Anti-Rabbit (green) at a dilution of 1:50 (1 hour at RT).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KCNJ2 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with KCNJ2 monoclonal antibody (Product # MA5-27681) using a dilution of 1:1000 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.