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LearnA300-856A
antibody from Invitrogen Antibodies
Targeting: DHX37
DDX37, Dhr1, KIAA1517, MGC2695, MGC4322
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- A300-856A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHX37 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DHX37 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-DHX37 antibody A300-856A (lot A300-856A-2) used for IP at 3 µg per reaction. DHX37 was also immunoprecipitated by a previous lot of this antibody (lot A300-856A-1) and rabbit anti-DHX37 antibody A300-857A. For blotting immunoprecipitated DHX37, A300-856A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DHX37 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX37 antibody A300-856A (lot A300-856A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
