sc-331

antibody from Santa Cruz Biotechnology

Targeting: NF2 ACN, BANF, merlin, SCH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-331

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-331, RRID:AB_2298548

Product name

Anti-NF2

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

A role for the retinoblastoma protein as a regulator of mouse osteoblast cell adhesion: implications for osteogenesis and osteosarcoma formation.

Sosa-García B, Gunduz V, Vázquez-Rivera V, Cress WD, Wright G, Bian H, Hinds PW, Santiago-Cardona PG
PloS one 2010 Nov 11;5(11):e13954

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Nf2/Merlin controls progenitor homeostasis and tumorigenesis in the liver.

Benhamouche S, Curto M, Saotome I, Gladden AB, Liu CH, Giovannini M, McClatchey AI
Genes & development 2010 Aug 15;24(16):1718-30

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Erbin and the NF2 tumor suppressor Merlin cooperatively regulate cell-type-specific activation of PAK2 by TGF-beta.

Wilkes MC, Repellin CE, Hong M, Bracamonte M, Penheiter SG, Borg JP, Leof EB
Developmental cell 2009 Mar;16(3):433-44

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Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.

Morris ZS, McClatchey AI
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9767-72

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Tissue-specific ablation of Prkar1a causes schwannomas by suppressing neurofibromatosis protein production.

Jones GN, Tep C, Towns WH 2nd, Mihai G, Tonks ID, Kay GF, Schmalbrock PM, Stemmer-Rachamimov AO, Yoon SO, Kirschner LS
Neoplasia (New York, N.Y.) 2008 Nov;10(11):1213-21

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Point mutation in the NF2 gene of HEI-193 human schwannoma cells results in the expression of a merlin isoform with attenuated growth suppressive activity.

Lepont P, Stickney JT, Foster LA, Meng JJ, Hennigan RF, Ip W
Mutation research 2008 Jan 1;637(1-2):142-51

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Localization to the cortical cytoskeleton is necessary for Nf2/merlin-dependent epidermal growth factor receptor silencing.

Cole BK, Curto M, Chan AW, McClatchey AI
Molecular and cellular biology 2008 Feb;28(4):1274-84

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Polyomavirus tumorantigens have a profound effect on gene expression in mouse fibroblasts

Britta Klucky, Birgit Koch, Martin Radolf, Peter Steinlein, Erhard Wintersberger
Oncogene 2004 May;23(27):4707-4721

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 65

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human hippocampus shows distinct nucleolar and moderate cytoplasmic positivity in neuronal cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.