Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319136 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NF2 phospho S518 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NF2 phospho S518 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NF2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by WB. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
- Validation comment
- WB
