711496

antibody from Invitrogen Antibodies

Targeting: NF2 ACN, BANF, merlin, SCH

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711496

Provider

Invitrogen Antibodies

Product name

NF2 Recombinant Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of NF2 was achieved by transfecting PC3 cells with NF2 specific siRNA (Silencer® select Product # s224112 and Product # s194647). Western blot analysis was performed using whole cell lysates from the NF2 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-NF2 Recombinant Rabbit Polyclonal Antibody (Product # 711496, 1-2 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Reduction of signal upon siRNA mediated knock down confirms that antibody is specific to NF2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of DU 145 (Lane 1), SH-SY5Y (Lane 2), U-2 OS (Lane 3), A-431 (Lane 4), PC-3 (Lane 5) and tissue extracts of Mouse Brain (Lane 6) and Rat Brain (Lane 7). The blots were probed with Anti-NF2 (Merlin) Recombinant Rabbit Polyclonal Antibody (Product # 711496, 1 µg/mL). A 70 kDa band corresponding to NF2 (Merlin) was observed across the cell lines and tissues tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).