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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-96130 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HeLa, 293T, HepG2; Cellular Location: Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane Immunogen sequence: PKTFTVRIVT MDAEMEFNCE MKWKGKDLFD LVCRTLGLRE TWFFGLQYTI KDTVAWLKMD KKVLDHDVSK EEPVTFHFLA KFYPENAEEE LVQEITQHLF FLQVKKQILD EKIYCPPEAS VLLASYAVQA KYGDYDPSVH KRGFLAQEEL LPKRVINLYQ MTPEMWEERI TAWYAEHRGR ARDEAEMEYL KIAQDLEMYG VNYFAIRNKK GTELLLGVDA LGLHIYDPEN RLTPKISFPW NEIRNISYSD KEFTIKPLDK KIDVFKFNSS KLRVNKLILQ LCIGNHDLFM RRRKADSLEV QQMKAQAREE KARKQMERQR LAREKQMREE AERTRDELER RLLQMKEEAT MANEALMRSE ETADLLAEKA QITEEEAKLL AQKAAEAEQE MQRIKATAIR TEEEKRLMEQ KVLEAEVLAL KMAEESERRA KEADQLKQDL QEAREAERRA KQKLLEIATK PTYPPMNPIP APLPPDIPSF NLIGDSLSFD FKDTDMKRLS MEIEKEKVEY MEKSKHLQEQ LNELKTEIEA LKLKERETAL DILHNENSDR GGSSKHNTIK KLTLQSAKSR VAFFEEL
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NF2 was performed in HeLa cells using NF2 Polyclonal Antibody (Product # PA5-96130).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NF2 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg NF2 Polyclonal antibody (Product # PA5-96130). Western blot was performed from the immunoprecipitate using NF2 Polyclonal antibody (Product # PA5-96130) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NF2 was performed in 200 µg extracts of HeLa cells using NF2 Polyclonal Antibody (Product # PA5-96130). Western blot was performed from the immunoprecipitate using NF2 Polyclonal Antibody at a dilution of 1:1000.
