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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-30170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFRS10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: JurKat. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (84%), Xenopus laevis (95%), Pig (100%), Chicken (99%), Sheep (99%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SFRS10 Polyclonal Antibody (Product # PA5-30170). Sample (30 µg of whole cell lysate). Lane A: JurKat. 12% SDS PAGE. SFRS10 Polyclonal Antibody (Product # PA5-30170) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SFRS10 was achieved by transfecting HeLa with SFRS10 specific siRNAs (Silencer® select Product # s12750, s12749). Western blot analysis (Fig. a) was performed using whole cell extracts from the SFRS10 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SFRS10 Polyclonal Antibody (Product # PA5-30170, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SFRS10.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell lysates (1% SDS) of Jurkat (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), SK-OV-3 (Lane 4), PC-3 (Lane 5), DU 145 (Lane 6) and MCF-7 (Lane 7). The blot was probed with SRSF10 Polyclonal Antibody (Product # PA5-30170, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 39 kDa band corresponding to SRSF10 was observed across the panel tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using TRA2B (Product # PA5-30170) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SFRS10 Polyclonal Antibody detects TRA2B protein at nucleus on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SFRS10 Polyclonal Antibody (Product # PA5-30170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SFRS10 Polyclonal Antibody detects TRA2B protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SFRS10 Polyclonal Antibody (Product # PA5-30170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
