LS-C674114

antibody from LSBio

Targeting: AACS ACSF1, FLJ12389, SUR-5

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C674114

Provider

LSBio

Product name

AACS Antibody LS-C674114

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in HEK293 whole cell lysate. All lanes: AACS antibody at 2.5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76, 68, 31 KDa. Observed band size: 76 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating AACS in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of AACS Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AACS Antibody (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating AACS in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of AACS Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AACS Antibody (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating AACS in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of AACS Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AACS Antibody (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)