- PA1-049 - Invitrogen Antibodies ADRB1 antibody

Submitted references Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity.

Osorio-Conles Ó, Olbeyra R, Moizé V, Ibarzabal A, Giró O, Viaplana J, Jiménez A, Vidal J, de Hollanda A
Nutrients 2022 Jun 24;14(13)

Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.

Funato Y, Yamazaki D, Okuzaki D, Yamamoto N, Miki H
Nature communications 2021 Jun 17;12(1):3683

Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice.

Lee HJ, Jin BY, Park MR, Seo KS, Jeong YT, Choi SH, Kim DH
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2021 Jul 1;25(4):355-363

Cardiomyocyte slowly activating delayed rectifier potassium channel: regulation by exercise and β-adrenergic signaling.

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Journal of applied physiology (Bethesda, Md. : 1985) 2020 May 1;128(5):1177-1185

Aldosterone Jeopardizes Myocardial Insulin and β-Adrenergic Receptor Signaling via G Protein-Coupled Receptor Kinase 2.

Cannavo A, Marzano F, Elia A, Liccardo D, Bencivenga L, Gambino G, Perna C, Rapacciuolo A, Cittadini A, Ferrara N, Paolocci N, Koch WJ, Rengo G
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Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line.

Tyurin-Kuzmin PA, Chechekhin VI, Ivanova AM, Dyikanov DT, Sysoeva VY, Kalinina NI, Tkachuk VA
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Atrial arrhythmias and autonomic dysfunction in rats exposed to chronic intermittent hypoxia.

Bober SL, Ciriello J, Jones DL
American journal of physiology. Heart and circulatory physiology 2018 Jun 1;314(6):H1160-H1168

Upregulation of β1-adrenoceptors is involved in the formation of gastric dysmotility in the 6-hydroxydopamine rat model of Parkinson's disease.

Song J, Zheng L, Zhang X, Feng X, Fan R, Sun L, Hong F, Zhang Y, Zhu J
Translational research : the journal of laboratory and clinical medicine 2014 Jul;164(1):22-31

Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways.

Rudolf R, Bogomolovas J, Strack S, Choi KR, Khan MM, Wagner A, Brohm K, Hanashima A, Gasch A, Labeit D, Labeit S
Age (Dordrecht, Netherlands) 2013 Oct;35(5):1663-74

Heterogeneous ventricular sympathetic innervation, altered beta-adrenergic receptor expression, and rhythm instability in mice lacking the p75 neurotrophin receptor.

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Adrenergic receptor agonists prevent bile duct injury induced by adrenergic denervation by increased cAMP levels and activation of Akt.

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Infusion of the beta-adrenergic blocker esmolol attenuates myocardial dysfunction in septic rats.

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Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.

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Neuroscience research 2001 Jun;40(2):155-62

Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.

Nakadate K, Imamura K, Watanabe Y
Neuroscience research 2001 Jun;40(2):155-62

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Tang C, Yang J, Wu LL, Dong LW, Liu MS
The American journal of physiology 1998 Apr;274(4 Pt 2):R1078-86

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ADR-Beta-1 was performed by loading 25 µg of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product # 34077).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunolocalization of B1AR in mouse kidney distal tubule using Product # PA1-049.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunolocalization of B1AR in mouse kidney distal tubule using Product # PA1-049.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 MuRF1 interacts with AChR on endo/lysosomal carriers. a BGT-biotin or solvent was injected into gastrocnemius muscles. Five hours later, muscles were collected and lysates prepared. From these, BGT-biotin-bound AChR and its interaction partners were affinity-precipitated with neutravidin beads. Panels show Western blot bands of lysates and affinity precipitates ( AP ) upon exposure to antibodies against AChRalpha, MuRF1, alpha-actinin (positive control), or beta1-adrenergic receptor ( beta1-AR , negative control), as indicated. b - c Tibialis anterior muscles were transfected with MuRF1-GFP. Ten days later, muscles were injected with the fluorescent AChR marker, BGT-AF647, and then monitored with in vivo confocal imaging. After acquisition, all images were electronically contrasted to highlight the weak endocytic AChR signals. In overlay pictures, AChR and GFP signals appear red and green , respectively. Colocalization of AChR and GFP signals is depicted in yellow. b Median filtered single optical planes of fluorescence signals. Scale bar represents 20 mum. c Panels depict high power views of the boxed regions shown in b . White and red arrowheads indicate colocalizing and partially overlapping signals, respectively, in endocytic carriers

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis in VAT and representative photomicrographs at magnification x10 of: ( A ) ADRB1, ( B ) UCP2 and ( C ) UCP3. The counterstaining of nuclei (Hoescht) is shown in blue and PLIN1 in red. Quantifications are presented as the average fluorescence intensity +- SD and compared by Student's t -test. * = p < 0.05, ** = p < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Immunofluorescent staining of adrenergic receptors in hTERT-MSCs ( A ) and primary adMSCs ( B ). Representative images of immunofluorescent staining of particular adrenergic receptors isoforms and corresponding bright field images. Receptor isoform name is marked in upper-left corner of each immunofluorescent image. Mouse IgG controls are the same as rabbit IgG and are not shown. Scale bar 50 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 In vivo effects of chronic aldosterone infusion on murine myocardium. (A) Representative immunoblots (upper panels) and densitometric quantitative analysis (lower panel) showing levels of GRK2 in total cardiac lysates from mice treated with Aldosterone (Aldo, 2 ug*mouse*day -1 ; n = 5) or Vehicle (PBS and EtOH 5%; n = 4) for 4 weeks. GAPDH levels were used as loading control; Mann-Whitney test. * p < 0.05 vs Vehicle. (B-C) Representative immunoblots (upper panels) and quantitative data showing levels of (B) phosphorylated IRS1, at serine 307 ( s307 pIRS1) and (C) beta1-adrenergic receptor (beta1AR) in total cardiac lysates from NLC and cardiac GRK2KO mice either treated with Aldo (2 ug*mouse*day -1 ; n = 4) or Vehicle (PBS and EtOH 5%; n = 3) for 4 weeks. GAPDH levels were used as loading control; Dunnet's post hoc test. * p < 0.05 vs NLC Vehicle.

PA1-049

Antibody data