Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 695602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#695602, RRID:AB_2650879
- Product name
- Purified anti-BACH2
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 16B10B53
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysates (15µg protein) from THP1 (negative control), Ramos and Raji (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-BACH2 antibody (clone 16B10B53) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M*: MW ladder with longer exposure time.
- Conjugate
- Unconjugated