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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-27953 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Jurkat. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (92%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EBP1 Polyclonal Antibody (Product # PA5-27953). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: Jurkat. 10% SDS PAGE. EBP1 Polyclonal Antibody (Product # PA5-27953) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EBP1 Polyclonal Antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 µg of EBP1 Polyclonal Antibody (Product # PA5-27953) 7.5% SDS-PAGE The immunoprecipitated EBP1 protein was detected by EBP1 Polyclonal Antibody (Product # PA5-27953) diluted at 1:1,000.
