PA5-29795

antibody from Invitrogen Antibodies

Targeting: CYLD CYLD1, KIAA0849, USPL2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29795

Provider

Invitrogen Antibodies

Product name

CYLD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (95%), Rat (94%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1.89 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cylindromatosis 1 in methanol-fixed HeLa cells using a Cylindromatosis 1 polyclonal antibody (Product # PA5-29795) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CYLD was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 100% Acetone for 5 minutes, at -20 degree Celsius and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CYLD Polyclonal Antibody (Product # PA5-29795) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to the cell membrane. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CYLD was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 100% Acetone for 5 minutes, at -20 degree Celsius and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CYLD Polyclonal Antibody (Product # PA5-29795) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to the cell membrane. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.