17721-1-AP

antibody from Proteintech Group

Targeting: DHX9 DDX9, LKP, RHA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17721-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17721-1-AP, RRID:AB_2092506

Product name

DHX9 antibody

Antibody type

Polyclonal

Description

DHX9 antibody (Cat. #17721-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

LncRNA CARMN suppresses EMT through inhibiting transcription of MMP2 activated by DHX9 in breast cancer.

Liao H, Wang H, Zheng R, Yu Y, Zhang Y, Lv L, Zhang B, Chen J
Cellular signalling 2024 Jan;113:110943

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DExH-box helicase 9 modulates hippocampal synapses and regulates neuropathic pain.

Yang L, Liu Q, Zhao Y, Lin N, Huang Y, Wang Q, Yang K, Wei R, Li X, Zhang M, Hao L, Wang H, Pan Z
iScience 2024 Feb 16;27(2):109016

---

A mutation in the low-complexity domain of splicing factor hnRNPA1 linked to amyotrophic lateral sclerosis disrupts distinct neuronal RNA splicing networks.

Lee YJ, Rio DC
Genes & development 2024 Feb 13;38(1-2):11-30

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CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair.

Lin L, Hu P, Luo M, Chen X, Xiao M, Zhong Z, Peng S, Chen G, Yang G, Zhang F, Zhang Y
International journal of biological macromolecules 2024 Apr;264(Pt 2):130541

---

Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner.

Long F, Li L, Xie C, Ma M, Wu Z, Lu Z, Liu B, Yang M, Zhang F, Ning Z, Zhong C, Yu B, Liu S, Wan L, Tian B, Yang K, Guo Y, Chen M, Chou J, Li X, Hu G, Lin C, Zhang Y
International journal of biological sciences 2023;19(12):3781-3803

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A pan-cancer analysis of the expression and molecular mechanism of DHX9 in human cancers.

Wang Y, Guo Y, Song Y, Zou W, Zhang J, Yi Q, Xiao Y, Peng J, Li Y, Yao L
Frontiers in pharmacology 2023;14:1153067

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DHX9 Strengthens Atherosclerosis Progression By Promoting Inflammation in Macrophages.

Huangfu N, Ma H, Tian M, Zhang J, Wang Y, Li Z, Chen X, Cui H
Inflammation 2023 Oct;46(5):1725-1738

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Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape.

Cao Y, Wu J, Hu Y, Chai Y, Song J, Duan J, Zhang S, Xu X
Cell reports 2023 Oct 31;42(10):113262

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PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.

Ye H, Shi W, Yang J, Wang L, Jiang X, Zhao H, Qin L, Qin J, Li L, Cai W, Guan J, Yang H, Zhou H, Yu Z, Sun H, Jiao Z
Cancer research 2023 Nov 15;83(22):3767-3782

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HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression.

Refaat AM, Nakata M, Husain A, Kosako H, Honjo T, Begum NA
Cell reports 2023 Mar 28;42(3):112284

---

NKAP acts with HDAC3 to prevent R-loop associated genome instability.

Zhang X, Duan J, Li Y, Jin X, Wu C, Yang X, Lu W, Ge W
Cell death and differentiation 2023 Jul;30(7):1811-1828

---

A New Cellular Interactome of SARS-CoV-2 Nucleocapsid Protein and Its Biological Implications.

Min YQ, Huang M, Feng K, Jia Y, Sun X, Ning YJ
Molecular & cellular proteomics : MCP 2023 Jul;22(7):100579

---

Alu RNA fold links splicing with signal recognition particle proteins.

Borovská I, Vořechovský I, Královičová J
Nucleic acids research 2023 Aug 25;51(15):8199-8216

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RNA-binding protein DHX9 promotes glioma growth and tumor-associated macrophages infiltration via TCF12.

Liu L, Zhou X, Cheng S, Ge Y, Chen B, Shi J, Li H, Li S, Li Y, Yuan J, Wu A, Liu X, Huang S, Xu Z, Dong J
CNS neuroscience & therapeutics 2023 Apr;29(4):988-999

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High Levels of DEAH-Box Helicases Relate to Poor Prognosis and Reduction of DHX9 Improves Radiosensitivity of Hepatocellular Carcinoma.

Chen X, Lin L, Chen G, Yan H, Li Z, Xiao M, He X, Zhang F, Zhang Y
Frontiers in oncology 2022;12:900671

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PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.

Dobrikov MI, Dobrikova EY, McKay ZP, Kastan JP, Brown MC, Gromeier M
mBio 2022 Jun 28;13(3):e0085422

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TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway.

Choudhury NR, Trus I, Heikel G, Wolczyk M, Szymanski J, Bolembach A, Dos Santos Pinto RM, Smith N, Trubitsyna M, Gaunt E, Digard P, Michlewski G
Nucleic acids research 2022 Jul 8;50(12):7097-7114

---

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Matkovic R, Morel M, Lanciano S, Larrous P, Martin B, Bejjani F, Vauthier V, Hansen MMK, Emiliani S, Cristofari G, Gallois-Montbrun S, Margottin-Goguet F
Nature communications 2022 Jan 10;13(1):66

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Long noncoding RNA SH3PXD2A-AS1 promotes NSCLC proliferation and accelerates cell cycle progression by interacting with DHX9.

Zhou Y, Yong H, Cui W, Chu S, Li M, Li Z, Bai J, Zhang H
Cell death discovery 2022 Apr 11;8(1):192

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SNORA42 promotes oesophageal squamous cell carcinoma development through triggering the DHX9/p65 axis.

Shan Y, Wei S, Xiang X, Dai S, Cui W, Zhao R, Zhang C, Zhang W, Zhao L, Shan B
Genomics 2021 Sep;113(5):3015-3029

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RNA pull-down confocal nanoscanning (RP-CONA) detects quercetin as pri-miR-7/HuR interaction inhibitor that decreases α-synuclein levels.

Zhu S, Choudhury NR, Rooney S, Pham NT, Koszela J, Kelly D, Spanos C, Rappsilber J, Auer M, Michlewski G
Nucleic acids research 2021 Jun 21;49(11):6456-6473

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Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Khan M, Hou S, Azam S, Lei H
Nucleic acids research 2021 Jun 21;49(11):6420-6436

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Interaction of 7SK with the Smn complex modulates snRNP production.

Ji C, Bader J, Ramanathan P, Hennlein L, Meissner F, Jablonka S, Mann M, Fischer U, Sendtner M, Briese M
Nature communications 2021 Feb 24;12(1):1278

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Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11

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LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification.

Hou P, Meng S, Li M, Lin T, Chu S, Li Z, Zheng J, Gu Y, Bai J
Journal of experimental & clinical cancer research : CR 2021 Feb 1;40(1):52

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High expression of DHX9 promotes the growth and metastasis of hepatocellular carcinoma.

Shi F, Cao S, Zhu Y, Yu Q, Guo W, Zhang S
Journal of clinical laboratory analysis 2021 Dec;35(12):e24052

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SPOP attenuates migration and invasion of choriocarcinoma cells by promoting DHX9 degradation.

Yuan D, Chen Y, Yang Z, Li G, Wu M, Jiang J, Li D, Yu Q
American journal of cancer research 2020;10(8):2428-2445

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R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.

Li Y, Song Y, Xu W, Li Q, Wang X, Li K, Wang J, Liu Z, Velychko S, Ye R, Xia Q, Wang L, Guo R, Dong X, Zheng Z, Dai Y, Li H, Yao M, Xue Y, Schöler HR, Sun Q, Yao H
Science advances 2020 Jun;6(24):eaba0777

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Splicing Regulator p54(nrb) /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma.

Hu Z, Dong L, Li S, Li Z, Qiao Y, Li Y, Ding J, Chen Z, Wu Y, Wang Z, Huang S, Gao Q, Zhao Y, He X
Hepatology (Baltimore, Md.) 2020 Aug;72(2):548-568

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Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J
Nature communications 2019 Dec 2;10(1):5492

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Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Azam S, Hou S, Zhu B, Wang W, Hao T, Bu X, Khan M, Lei H
RNA biology 2019 Aug;16(8):1001-1009

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Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.

Kooshapur H, Choudhury NR, Simon B, Mühlbauer M, Jussupow A, Fernandez N, Jones AN, Dallmann A, Gabel F, Camilloni C, Michlewski G, Caceres JF, Sattler M
Nature communications 2018 Jun 26;9(1):2479

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RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.

Choudhury NR, Heikel G, Trubitsyna M, Kubik P, Nowak JS, Webb S, Granneman S, Spanos C, Rappsilber J, Castello A, Michlewski G
BMC biology 2017 Nov 8;15(1):105

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Minichromosome maintenance protein 2 and 3 promote osteosarcoma progression via DHX9 and predict poor patient prognosis.

Cheng DD, Zhang HZ, Yuan JQ, Li SJ, Yang QC, Fan CY
Oncotarget 2017 Apr 18;8(16):26380-26393

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Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 To Regulate Viral RNA Synthesis.

Liu L, Tian J, Nan H, Tian M, Li Y, Xu X, Huang B, Zhou E, Hiscox JA, Chen H
Journal of virology 2016 Jun 1;90(11):5384-5398

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Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation.

Choudhury NR, Nowak JS, Zuo J, Rappsilber J, Spoel SH, Michlewski G
Cell reports 2014 Nov 20;9(4):1265-72

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Lin28a regulates neuronal differentiation and controls miR-9 production.

Nowak JS, Choudhury NR, de Lima Alves F, Rappsilber J, Michlewski G
Nature communications 2014 Apr 11;5:3687

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17721-1-AP(DHX9 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DHX9 antibody 17721-1-AP at 1:100 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 17721-1-AP(DHX9 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 17721-1-AP(DHX9 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue