Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB110-40579 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40579, RRID:AB_669072
- Product name
- Rabbit Polyclonal RNA Helicase A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antigen sequence
The epitope recognized by NB 110-40
578 maps to a region between residu
e 1220 and the C-terminus (residue
1270) of human DEAH (Asp-Glu-Ala-Hi
s) Box Polypeptide 9 using the numb
ering given in entry CAA71668.1 (Ge
neID 1660).- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Poxviral protein E3-altered cytokine production reveals that DExD/H-box helicase 9 controls Toll-like receptor-stimulated immune responses.
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.
Chromatin context and ncRNA highlight targets of MeCP2 in brain.
Identification of small molecules that suppress microRNA function and reverse tumorigenesis.
Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.
Dempsey A, Keating SE, Carty M, Bowie AG
The Journal of biological chemistry 2018 Sep 28;293(39):14989-15001
The Journal of biological chemistry 2018 Sep 28;293(39):14989-15001
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.
Moldovan JB, Moran JV
PLoS genetics 2015 May;11(5):e1005121
PLoS genetics 2015 May;11(5):e1005121
Chromatin context and ncRNA highlight targets of MeCP2 in brain.
Maxwell SS, Pelka GJ, Tam PP, El-Osta A
RNA biology 2013 Nov;10(11):1741-57
RNA biology 2013 Nov;10(11):1741-57
Identification of small molecules that suppress microRNA function and reverse tumorigenesis.
Watashi K, Yeung ML, Starost MF, Hosmane RS, Jeang KT
The Journal of biological chemistry 2010 Aug 6;285(32):24707-16
The Journal of biological chemistry 2010 Aug 6;285(32):24707-16
Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.
Lawrence P, Rieder E
Journal of virology 2009 Nov;83(21):11356-66
Journal of virology 2009 Nov;83(21):11356-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 on HeLa whole cell lystate using NB110-40579.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1 For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB