TA500381

antibody from OriGene

Targeting: TRPM4 FLJ20041

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500381

Provider

OriGene

Proper citation

Product name

TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)

Antibody type

Monoclonal

Description

TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

TRPM4

Isotype

IgG

Antibody clone number

OTI10H5

Vial size

100 µl

Concentration

0.77 mg/ml

References

Submitted references

Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel.

Sarmiento D, Montorfano I, Cerda O, Cáceres M, Becerra A, Cabello-Verrugio C, Elorza AA, Riedel C, Tapia P, Velásquez LA, Varela D, Simon F
Microvascular research 2015 Mar;98:187-96

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Transient receptor potential melastatin 4 inhibition prevents lipopolysaccharide-induced endothelial cell death.

Becerra A, Echeverría C, Varela D, Sarmiento D, Armisén R, Nuñez-Villena F, Montecinos M, Simon F
Cardiovascular research 2011 Sep 1;91(4):677-84

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (RC216888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure from citation: Western Blot of TRPM4 protein level by using anti-TRPM4 antibody in human endothelial cells.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)

Validation comment

IHC