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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-30289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ST3GAL5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Jurkat, Raji, mouse brain, U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ST3GAL5 Polyclonal Antibody (Product # PA5-30289). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. ST3GAL5 Polyclonal Antibody (Product # PA5-30289) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ST3GAL5 antibody detects ST3GAL5 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ST3GAL5 antibody ST3GAL5 Polyclonal Antibody (Product # PA5-30289) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ST3GAL5 Polyclonal Antibody (Product # PA5-30289). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. ST3GAL5 Polyclonal Antibody (Product # PA5-30289) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using ST3GAL5 (Product # PA5-30289) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
