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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA325024 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal APG1 (ULK1) Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal APG1 (ULK1) Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ULK1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ULK1 Pab (Cat. #TA325024) in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine , then incubated with TA325024 APG1 (ULK1) (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
