PA5-109212

antibody from Invitrogen Antibodies

Targeting: ULK1 ATG1, ATG1A

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109212

Provider

Invitrogen Antibodies

Product name

ULK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, MCF-7, SH-SY5Y, rat heart tissue, human kidney tissue, human placenta tissue, mouse skeletal muscle tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ULK1 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ULK1 polyclonal antibody (Product # PA5-109212), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ULK1 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ULK1 polyclonal antibody (Product # PA5-109212), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ULK1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ULK1 polyclonal antibody (Product # PA5-109212), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ULK1 in paraffin-embedded human placenta tissue. Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ULK1 in paraffin-embedded rat heart tissue. Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ULK1 in paraffin-embedded mouse skeletal muscle tissue. Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ULK1 in paraffin-embedded human placenta tissue. Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ULK1 in paraffin-embedded human kidney tissue. Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ULK1 in SH-SY5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with ULK1 polyclonal antibody (Product # PA5-109212) at a dilution of 1:100.