ABIN1449620

antibody from antibodies-online

Targeting: ULK1 ATG1, ATG1A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN1449620

Provider

antibodies-online

Product name

anti-Unc-51 Like Kinase 1 (C. Elegans) (ULK1) (AA 649-679) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 649~679 amino acids from the center region of Human APG1 Genename: ULK1

Description

Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Reactivity

Human

Host

Rabbit

Epitope

AA 649-679

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Intensive expression of UNC-51-like kinase 1 is a novel biomarker of poor prognosis in patients with esophageal squamous cell carcinoma.

Jiang S, Li Y, Zhu YH, Wu XQ, Tang J, Li Z, Feng GK, Deng R, Li DD, Luo RZ, Zhang MF, Qin W, Wang X, Jia WH, Zhu XF
Cancer science 2011 Aug;102(8):1568-75

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Retraction: Blockage of intracellular proton extrusion with proton pump inhibitor induces apoptosis in gastric cancer.

Yeo M, Kim DK, Park HJ, Cho SW, Cheong JY, Lee KJ
Cancer science 2008 Jan;99(1):185

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Human ULK1, a novel serine/threonine kinase related to UNC-51 kinase of Caenorhabditis elegans: cDNA cloning, expression, and chromosomal assignment.

Kuroyanagi H, Yan J, Seki N, Yamanouchi Y, Suzuki Y, Takano T, Muramatsu M, Shirasawa T
Genomics 1998 Jul 1;51(1):76-85

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of anti-ULK1 Antibody Cat.-No  AP32188PU-N in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence