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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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- Product number
- LS-C380155 - Provider product page
- Provider
- LSBio
- Product name
- PIH1D1 Antibody LS-C380155
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: PIH1D1 antibody at 6 ug/ml. Lane 1: A431 whole cell lysate. Lane 2: A549 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating PIH1D1 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of PIH1D1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PIH1D1 Antibody (6µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating PIH1D1 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of PIH1D1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PIH1D1 Antibody (6µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at dilution of 1:100.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at dilution of 1:100.
