H00055011-M01

antibody from Abnova Corporation

Targeting: PIH1D1 FLJ20643, MOT48, NOP17, Pih1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00055011-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00055011-M01, RRID:AB_426000

Product name

FLJ20643 monoclonal antibody (M01), clone 4F5-1E10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FLJ20643.

Antigen sequence

MANPKLLGLELSEAEAIGADSARFEELLLQASKEL
QQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINIC
HSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGE
PHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRE
LVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSIS
QQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHL
NLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLV
MGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLM
VAMPLLLVPS


Isotype

IgG

Antibody clone number

4F5-1E10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PIH1D1 expression in transfected 293T cell line by FLJ20643 monoclonal antibody (M01), clone 4F5-1E10.Lane 1: PIH1D1 transfected lysate (Predicted MW: 32.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PIH1D1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of PIH1D1 transfected lysate using anti-PIH1D1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PIH1D1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol