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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-59653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FDX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NLDIDGFGAC EGTLACSTCH LIFEDHIYEK LDAITDEEND MLDLAYGLTD RSRLGC Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 96%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FDX1 in control (vector only transfected HEK293T lysate) and FDX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FDX1 Polyclonal Antibody (Product # PA5-59653).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FDX1 in human cell line A-431 shows positivity in mitochondria. Samples were probed using a FDX1 Polyclonal Antibody (Product # PA5-59653).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in human colon using FDX1 Polyclonal Antibody (Product # PA5-59653).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in human lymph node using FDX1 Polyclonal Antibody (Product # PA5-59653).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in adrenal gland using a FDX1 Polyclonal Antibody (Product # PA5-59653) shows cytoplasmic positivity in cortical cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in human adrenal gland, cerebral cortex, colon and lymph node using FDX1 Polyclonal Antibody (Product # PA5-59653) (A) shows similar protein distribution across tissues to an independent FDX1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in human adrenal gland using FDX1 Polyclonal Antibody (Product # PA5-59653) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FDX1 in human cerebral cortex using FDX1 Polyclonal Antibody (Product # PA5-59653) shows low expression as expected.
