12592-1-AP

antibody from Proteintech Group

Targeting: FDX1 ADX, FDX

Provider product page for 12592-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 12592-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12592-1-AP, RRID:AB_11182486
Product name: FDX1 antibody
Antibody type: Polyclonal
Description: KD/KO validated FDX1 antibody (Cat. #12592-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

FDX1 protein structure - 12592-1-AP shown in red.

Submitted references GSH exhaustion via inhibition of xCT-GSH-GPX4 pathway synergistically enhanced DSF/Cu-induced cuproptosis in myelodysplastic syndromes.

Li H, Li Y, Yu Y, Ren X, Yang C, Jin W, Li K, Zhou Y, Wu C, Shen Y, Hu W, Liu Y, Yu L, Tong X, Du J, Wang Y
Free radical biology & medicine 2024 Sep;222:130-148

SP1/CTR1-mediated oxidative stress-induced cuproptosis in intervertebral disc degeneration.

Chen X, Li K, Xiao Y, Wu W, Lin H, Qing X, Tian S, Liu S, Feng S, Wang B, Shao Z, Peng Y
BioFactors (Oxford, England) 2024 Sep-Oct;50(5):1009-1023

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Wang X, Shi Y, Shi H, Liu X, Liao A, Liu Z, Orlowski RZ, Zhang R, Wang H
Journal of experimental & clinical cancer research : CR 2024 Mar 5;43(1):68

A cuproptosis-related gene expression signature predicting clinical prognosis and immune responses in intrahepatic cholangiocarcinoma detected by single-cell RNA sequence analysis.

Ren H, Liu C, Zhang C, Wu H, Zhang J, Wang Z, Chen L, Wang H, Shao C, Zhou L
Cancer cell international 2024 Mar 2;24(1):92

Integrated profiling identifies ferredoxin 1 as an immune-related biomarker of malignant phenotype in glioma.

Xie D, Huang H, Guo Y, Jiang Z, Kuang Y, Huang H, Liu W, Wang L, Xin Z, Wang B, Ren C, Jiang X
Heliyon 2024 Mar 15;10(5):e26976

Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer.

Chong W, Ren H, Chen H, Xu K, Zhu X, Liu Y, Sang Y, Li H, Liu J, Ye C, Shang L, Jing C, Li L
iMeta 2024 Jun;3(3):e190

Elesclomol-copper synergizes with imidazole ketone erastin by promoting cuproptosis and ferroptosis in myelodysplastic syndromes.

Gao Y, Jin F, Zhang P, Zheng C, Zheng X, Xie J, Lu Y, Tong X, Du J, Zhang J, Wang Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jun;175:116727

Mitochondrial-Targeted Copper Delivery for Cuproptosis-Based Synergistic Cancer Therapy.

Deng J, Zhuang H, Shao S, Zeng X, Xue P, Bai T, Wang X, Shangguan S, Chen Y, Yan S, Huang W
Advanced healthcare materials 2024 Jul;13(18):e2304522

Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis.

Zhang P, Zhou C, Ren X, Jing Q, Gao Y, Yang C, Shen Y, Zhou Y, Hu W, Jin F, Xu H, Yu L, Liu Y, Tong X, Li Y, Wang Y, Du J
Redox biology 2024 Feb;69:103007

Combination of the cuproptosis inducer disulfiram and anti‑PD‑L1 abolishes NSCLC resistance by ATP7B to regulate the HIF‑1 signaling pathway.

Li P, Sun Q, Bai S, Wang H, Zhao L
International journal of molecular medicine 2024 Feb;53(2)

Deep learning enables the discovery of a novel cuproptosis-inducing molecule for the inhibition of hepatocellular carcinoma.

Yang F, Jia L, Zhou HC, Huang JN, Hou MY, Liu FT, Prabhu N, Li ZJ, Yang CB, Zou C, Nordlund P, Wang JG, Dai LY
Acta pharmacologica Sinica 2024 Feb;45(2):391-404

Copper-coordinated nanoassemblies based on photosensitizer-chemo prodrugs and checkpoint inhibitors for enhanced apoptosis-cuproptosis and immunotherapy.

Liang W, Han C, Zhang D, Liu C, Zhu M, Xu F, Fang C, Zhang S, Liu C, Li Y
Acta biomaterialia 2024 Feb;175:341-352

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers.

Liu C, Chen L, Cong Y, Cheng L, Shuai Y, Lv F, Chen K, Song Y, Xing Y
Cell death & disease 2024 Feb 16;15(2):149

Novel immune cross-talk between inflammatory bowel disease and IgA nephropathy.

Yan Q, Zhao Z, Liu D, Li J, Pan S, Duan J, Liu Z
Renal failure 2024 Dec;46(1):2337288

Copper Homeostasis Based on Cuproptosis-Related Signature Optimizes Molecular Subtyping and Treatment of Glioma.

Zhang S, Yu H, Sun S, Fan X, Bi W, Li S, Wang W, Fang Z, Chen X
Molecular neurobiology 2024 Aug;61(8):4962-4975

Cellular Trojan Horse initiates bimetallic Fe-Cu MOF-mediated synergistic cuproptosis and ferroptosis against malignancies.

Chen K, Zhou A, Zhou X, He J, Xu Y, Ning X
Science advances 2024 Apr 12;10(15):eadk3201

Identification of core cuprotosis-correlated biomarkers in abdominal aortic aneurysm immune microenvironment based on bioinformatics.

Hu J, Xue S, Xu Z, Wu Z, Xu X, Wang X, Liu G, Lu X, Li B, Liu X
Frontiers in immunology 2023;14:1138126

Identification of cuproptosis-related subtypes and the development of a prognostic model in glioma.

Wu Z, Li W, Zhu H, Li X, Zhou Y, Chen Q, Huang H, Zhang W, Jiang X, Ren C
Frontiers in genetics 2023;14:1124439

Identification of a cuproptosis and copper metabolism gene-related lncRNAs prognostic signature associated with clinical and immunological characteristics of hepatocellular carcinoma.

Yuan W, Xiao JH, Zhang JS, Mao BL, Wang PZ, Wang BL
Frontiers in oncology 2023;13:1153353

ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer.

Zhu X, Chen H, Li H, Ren H, Ye C, Xu K, Liu J, Du F, Zhang Z, Liu Y, Xie X, Wang M, Ma T, Chong W, Shang L, Li L
Frontiers in oncology 2023;13:1115510

Lipoylation is dependent on the ferredoxin FDX1 and dispensable under hypoxia in human cells.

Joshi PR, Sadre S, Guo XA, McCoy JG, Mootha VK
The Journal of biological chemistry 2023 Sep;299(9):105075

Evaluation the role of cuproptosis-related genes in the pathogenesis, diagnosis and molecular subtypes identification of atherosclerosis.

Wang M, Cheng L, Xiang Q, Gao Z, Ding Y, Xie H, Chen X, Yu P, Shen L
Heliyon 2023 Oct;9(10):e21158

Lactylation of METTL16 promotes cuproptosis via m(6)A-modification on FDX1 mRNA in gastric cancer.

Sun L, Zhang Y, Yang B, Sun S, Zhang P, Luo Z, Feng T, Cui Z, Zhu T, Li Y, Qiu Z, Fan G, Huang C
Nature communications 2023 Oct 20;14(1):6523

Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.

Xing T, Li L, Chen Y, Ju G, Li G, Zhu X, Ren Y, Zhao J, Cheng Z, Li Y, Xu D, Liang J
Cell reports. Medicine 2023 Nov 21;4(11):101264

4-Octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to promote cuproptosis in colorectal cancer.

Yang W, Wang Y, Huang Y, Yu J, Wang T, Li C, Yang L, Zhang P, Shi L, Yin Y, Tao K, Li R
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Mar;159:114301

FDX1-dependent and independent mechanisms of elesclomol-mediated intracellular copper delivery.

Zulkifli M, Spelbring AN, Zhang Y, Soma S, Chen S, Li L, Le T, Shanbhag V, Petris MJ, Chen TY, Ralle M, Barondeau DP, Gohil VM
Proceedings of the National Academy of Sciences of the United States of America 2023 Mar 7;120(10):e2216722120

A novel cuproptosis-related gene signature to predict prognosis in Glioma.

Zhang M, Liu X, Wang D, Ruan X, Wang P, Liu L, Xue Y
BMC cancer 2023 Mar 13;23(1):237

Expression of Ferredoxin1 in cisplatin‑resistant ovarian cancer cells confers their resistance against ferroptosis induced by cisplatin.

Takahashi R, Kamizaki K, Yamanaka K, Terai Y, Minami Y
Oncology reports 2023 Jun;49(6)

Critical role of antioxidant programs in enzalutamide-resistant prostate cancer.

Blatt EB, Parra K, Neeb A, Buroni L, Bogdan D, Yuan W, Gao Y, Gilbreath C, Paschalis A, Carreira S, DeBerardinis RJ, Mani RS, de Bono JS, Raj GV
Oncogene 2023 Jul;42(30):2347-2359

Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting.

Cai C, Zhou K, Jing J, Ren Y, Weng G, Cen D, Wang X, Huang S
Aging 2023 Jul 10;15(13):6117-6134

Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells.

Basque A, Touaibia M, Martin LJ
Toxicology in vitro : an international journal published in association with BIBRA 2023 Feb;86:105505

SP1 impacts the primordial to primary follicle transition by regulating cholesterol metabolism in granulosa cells.

Zhou J, Lin L, Cai H, Liu L, Wang H, Zhang J, Xia G, Wang J, Wang F, Wang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Feb;37(2):e22767

Glutathione ameliorates the meiotic defects of copper exposed ovine oocytes via inhibiting the mitochondrial dysfunctions.

Ren J, Wang B, Li L, Li S, Ma Y, Su L, Liu G, Liu Y, Dai Y
Ecotoxicology and environmental safety 2023 Feb;251:114530

Bioinformatics analysis and experimental validation of cuproptosis-related lncRNA LINC02154 in clear cell renal cell carcinoma.

Shen J, Wang L, Bi J
BMC cancer 2023 Feb 16;23(1):160

Dexmedetomidine enables copper homeostasis in cerebral ischemia/reperfusion via ferredoxin 1.

Guo Q, Ma M, Yu H, Han Y, Zhang D
Annals of medicine 2023 Dec;55(1):2209735

Integrated bioinformatics and experiment revealed that cuproptosis is the potential common pathogenesis of three kinds of primary cardiomyopathy.

Wang M, Xu X, Li J, Gao Z, Ding Y, Chen X, Xiang Q, Shen L
Aging 2023 Dec 11;15(23):14210-14241

FDX1 Is Required for the Biogenesis of Mitochondrial Cytochrome c Oxidase in Mammalian Cells.

Zulkifli M, Okonkwo AU, Gohil VM
Journal of molecular biology 2023 Dec 1;435(23):168317

Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer.

Li J, Yu T, Sun J, Zeng Z, Liu Z, Ma M, Zheng Z, He Y, Kang W
Aging 2023 Apr 7;15(7):2772-2796

A Novel Cuprotosis-Related Gene FDX1 Signature for Overall Survival Prediction in Clear Cell Renal Cell Carcinoma Patients.

Zhang WT, Gong YM, Zhang CY, Pan JS, Huang T, Li YX
BioMed research international 2022;2022:9196540

FDX1 expression predicts favourable prognosis in clear cell renal cell carcinoma identified by bioinformatics and tissue microarray analysis.

Huang X, Wang T, Ye J, Feng H, Zhang X, Ma X, Wang B, Huang Y, Zhang X
Frontiers in genetics 2022;13:994741

Multi-omics pan-cancer study of cuproptosis core gene FDX1 and its role in kidney renal clear cell carcinoma.

Xu J, Hu Z, Cao H, Zhang H, Luo P, Zhang J, Wang X, Cheng Q, Li J
Frontiers in immunology 2022;13:981764

Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.

Yang L, Zhang Y, Wang Y, Jiang P, Liu F, Feng N
Frontiers in pharmacology 2022;13:938134

Role of cuproptosis-related gene in lung adenocarcinoma.

Liu Y, Lin W, Yang Y, Shao J, Zhao H, Wang G, Shen A
Frontiers in oncology 2022;12:1080985

Establishment and experimental validation of a novel cuproptosis-related gene signature for prognostic implication in cholangiocarcinoma.

Chen J, Yu X, Tong H, Tang C, Tang Z
Frontiers in oncology 2022;12:1054063

Xie M, Cheng B, Yu S, He Y, Cao Y, Zhou T, Han K, Dai R, Wang R
Cells 2022 Dec 31;12(1)

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Wang Y, Yen FS, Zhu XG, Timson RC, Weber R, Xing C, Liu Y, Allwein B, Luo H, Yeh HW, Heissel S, Unlu G, Gamazon ER, Kharas MG, Hite R, Birsoy K
Nature 2021 Nov;599(7883):136-140

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

Weber RA, Yen FS, Nicholson SPV, Alwaseem H, Bayraktar EC, Alam M, Timson RC, La K, Abu-Remaileh M, Molina H, Birsoy K
Molecular cell 2020 Feb 6;77(3):645-655.e7

Mitochondrial metabolism promotes adaptation to proteotoxic stress.

Tsvetkov P, Detappe A, Cai K, Keys HR, Brune Z, Ying W, Thiru P, Reidy M, Kugener G, Rossen J, Kocak M, Kory N, Tsherniak A, Santagata S, Whitesell L, Ghobrial IM, Markley JL, Lindquist S, Golub TR
Nature chemical biology 2019 Jul;15(7):681-689

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes.

Shao L, Chian RC, Xu Y, Yan Z, Zhang Y, Gao C, Gao L, Liu J, Cui Y
Reproduction, fertility, and development 2015 May 20;

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 12592-1-AP(FDX1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 12592-1-AP(FDX1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The FDX1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human FDX1. This antibody recognizes human,mouse,rat antigen. The FDX1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12592-1-AP.pdf

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