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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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- Product number
- TA500807 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500807, RRID:AB_11125458
- Product name
- GBE1 mouse monoclonal antibody, clone OTI4D9 (formerly 4D9)
- Antibody type
- Monoclonal
- Description
- GBE1 mouse monoclonal antibody, clone OTI4D9 (formerly 4D9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GBE1
- Isotype
- IgG
- Antibody clone number
- OTI4D9
- Vial size
- 100 µl
- Concentration
- 0.7 mg/ml
Submitted references Adult polyglucosan body disease: clinical and histological heterogeneity of a large Italian family.
Colombo I, Pagliarani S, Testolin S, Salsano E, Napoli LM, Bordoni A, Salani S, D'Adda E, Morandi L, Farina L, Magri F, Riva M, Prelle A, Sciacco M, Comi GP, Moggio M
Neuromuscular disorders : NMD 2015 May;25(5):423-8
Neuromuscular disorders : NMD 2015 May;25(5):423-8
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: WB, performed with anti-GBE1 antibody (GBE1 mousemonoclonal antibody TA500807, Origene), showed a decrease of protein on skeletal muscle of patients II-5 /II-6, being protein absent on cardiac muscle of a neonateaffected with the congenital variant of glycogenosis IV (GSD-IV or Andersen disease).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GBE1 mouse monoclonal antibody (TA500807) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody(TA500807),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
