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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA5-25028 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GBE1 Monoclonal Antibody (OTI1D11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1D11
- Vial size
- 100 µL
- Concentration
- 1.1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBE1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with GBE1 (Product # MA5-25028) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on HEK293T cells overexpressing GBE1. Antigen-antibody complexes were formed by incubating the sample with 2 µg of GBE1 monoclonal antibody (Product # MA5-25028) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBE1 in human tissue (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen) samples using 10 µg per lane. Samples were separated by SDS-PAGE and probed with GBE1 (Product # MA5-25028) monoclonal antibody at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBE1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with GBE1 (Product # MA5-25028) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBE1 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with GBE1 (Product # MA5-25028) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GBE1 in COS7 cells. Cells were transfected with a plasmid overexpressing GBE1 and probed with a GBE1 monoclonal antibody (Product # MA5-25028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GBE1 in COS7 cells. Cells were transfected with a plasmid overexpressing GBE1 and probed with a GBE1 monoclonal antibody (Product # MA5-25028).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GBE1 monoclonal antibody (Product # MA5-25028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GBE1 monoclonal antibody (Product # MA5-25028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GBE1 monoclonal antibody (Product # MA5-25028).
