LS-C784482

antibody from LSBio

Targeting: GBE1

Western blot

Antibody data

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Validation data

GBE1 protein structure - LS-C784482 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Kidney tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of prostate tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of prostate tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).