10179-1-AP

antibody from Proteintech Group

Targeting: IMMT HMP, Mic60, MICOS60, MINOS2, P87, P89

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10179-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10179-1-AP, RRID:AB_2127193

Product name

Mitofilin antibody

Antibody type

Polyclonal

Description

KD/KO validated Mitofilin antibody (Cat. #10179-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Oct;35(10):e21891

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Melatonin protects against excessive autophagy-induced mitochondrial and ovarian reserve function deficiency though ERK signaling pathway in Chinese hamster ovary (CHO) cells.

Xie QE, Wang MY, Cao ZP, Du X, Ji DM, Liang D, Cao YX, Liu YJ
Mitochondrion 2021 Nov;61:44-53

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Mt-Keima detects PINK1-PRKN mitophagy in vivo with greater sensitivity than mito-QC.

Liu YT, Sliter DA, Shammas MK, Huang X, Wang C, Calvelli H, Maric DS, Narendra DP
Autophagy 2021 Nov;17(11):3753-3762

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The TFAM-to-mtDNA ratio defines inner-cellular nucleoid populations with distinct activity levels.

Brüser C, Keller-Findeisen J, Jakobs S
Cell reports 2021 Nov 23;37(8):110000

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A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.

Latorre-Muro P, O'Malley KE, Bennett CF, Perry EA, Balsa E, Tavares CDJ, Jedrychowski M, Gygi SP, Puigserver P
Cell metabolism 2021 Mar 2;33(3):598-614.e7

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Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes.

Ma JY, Feng X, Xie FY, Li S, Chen LN, Luo SM, Yin S, Ou XH
Genetics 2021 Jun 24;218(2)

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Low abundance of Mfn2 protein correlates with reduced mitochondria-SR juxtaposition and mitochondrial cristae density in human men skeletal muscle: Examining organelle measurements from TEM images.

Castro-Sepulveda M, Fernández-Verdejo R, Tuñón-Suárez M, Morales-Zúñiga J, Troncoso M, Jannas-Vela S, Zbinden-Foncea H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21553

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High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy.

Edwards SJ, Carannante V, Kuhnigk K, Ring H, Tararuk T, Hallböök F, Blom H, Önfelt B, Brismar H
Frontiers in molecular biosciences 2020;7:208

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Parkin interacts with Mitofilin to increase dopaminergic neuron death in response to Parkinson's disease-related stressors.

Imam Aliagan AD, Ahwazi MD, Tombo N, Feng Y, Bopassa JC
American journal of translational research 2020;12(11):7542-7564

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ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation.

Qin J, Guo Y, Xue B, Shi P, Chen Y, Su QP, Hao H, Zhao S, Wu C, Yu L, Li D, Sun Y
Nature communications 2020 Sep 8;11(1):4471

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Cardiac ischemia/reperfusion stress reduces inner mitochondrial membrane protein (mitofilin) levels during early reperfusion.

Tombo N, Imam Aliagan AD, Feng Y, Singh H, Bopassa JC
Free radical biology & medicine 2020 Oct;158:181-194

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OPA1 and MICOS Regulate mitochondrial crista dynamics and formation.

Hu C, Shu L, Huang X, Yu J, Li L, Gong L, Yang M, Wu Z, Gao Z, Zhao Y, Chen L, Song Z
Cell death & disease 2020 Oct 31;11(10):940

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Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.

Liu YT, Huang X, Nguyen D, Shammas MK, Wu BP, Dombi E, Springer DA, Poulton J, Sekine S, Narendra DP
Human molecular genetics 2020 Jun 3;29(9):1547-1567

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β-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle.

Monsalves-Alvarez M, Morales PE, Castro-Sepulveda M, Sepulveda C, Rodriguez JM, Chiong M, Eisner V, Lavandero S, Troncoso R
Nutrients 2020 Jun 29;12(7)

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MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation.

Stephan T, Brüser C, Deckers M, Steyer AM, Balzarotti F, Barbot M, Behr TS, Heim G, Hübner W, Ilgen P, Lange F, Pacheu-Grau D, Pape JK, Stoldt S, Huser T, Hell SW, Möbius W, Rehling P, Riedel D, Jakobs S
The EMBO journal 2020 Jul 15;39(14):e104105

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Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Ayanlaja AA, Ji G, Wang J, Gao Y, Cheng B, Kanwore K, Zhang L, Xiong Y, Kambey PA, Gao D
Cell communication and signaling : CCS 2020 Feb 12;18(1):24

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Mitochondrial Safeguard: a stress response that offsets extreme fusion and protects respiratory function via flickering-induced Oma1 activation.

Murata D, Yamada T, Tokuyama T, Arai K, Quirós PM, López-Otín C, Iijima M, Sesaki H
The EMBO journal 2020 Dec 15;39(24):e105074

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Oncogenic human herpesvirus hijacks proline metabolism for tumorigenesis.

Choi UY, Lee JJ, Park A, Zhu W, Lee HR, Choi YJ, Yoo JS, Yu C, Feng P, Gao SJ, Chen S, Eoh H, Jung JU
Proceedings of the National Academy of Sciences of the United States of America 2020 Apr 7;117(14):8083-8093

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Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent.

Wolf DM, Segawa M, Kondadi AK, Anand R, Bailey ST, Reichert AS, van der Bliek AM, Shackelford DB, Liesa M, Shirihai OS
The EMBO journal 2019 Nov 15;38(22):e101056

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Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.

Violitzi F, Perivolidi VI, Thireou T, Grivas I, Haralambous S, Samiotaki M, Panayotou G, Douni E
Journal of proteome research 2019 Nov 1;18(11):3896-3912

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TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

Wang P, Deng J, Dong J, Liu J, Bigio EH, Mesulam M, Wang T, Sun L, Wang L, Lee AY, McGee WA, Chen X, Fushimi K, Zhu L, Wu JY
PLoS genetics 2019 May;15(5):e1007947

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ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes through the PERK-eIF2α Axis.

Balsa E, Soustek MS, Thomas A, Cogliati S, García-Poyatos C, Martín-García E, Jedrychowski M, Gygi SP, Enriquez JA, Puigserver P
Molecular cell 2019 Jun 6;74(5):877-890.e6

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MLKL Requires the Inositol Phosphate Code to Execute Necroptosis.

Dovey CM, Diep J, Clarke BP, Hale AT, McNamara DE, Guo H, Brown NW Jr, Cao JY, Grace CR, Gough PJ, Bertin J, Dixon SJ, Fiedler D, Mocarski ES, Kaiser WJ, Moldoveanu T, York JD, Carette JE
Molecular cell 2018 Jun 7;70(5):936-948.e7

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Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

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Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.

Straub IR, Janer A, Weraarpachai W, Zinman L, Robertson J, Rogaeva E, Shoubridge EA
Human molecular genetics 2018 Jan 1;27(1):178-189

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Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association.

Kelly FD, Wei BM, Cygan AM, Parker ML, Boulanger MJ, Boothroyd JC
mSphere 2017 May-Jun;2(3)

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Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria.

Steffen J, Vashisht AA, Wan J, Jen JC, Claypool SM, Wohlschlegel JA, Koehler CM
Molecular biology of the cell 2017 Mar 1;28(5):600-612

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Novel insights into SLC25A46-related pathologies in a genetic mouse model.

Terzenidou ME, Segklia A, Kano T, Papastefanaki F, Karakostas A, Charalambous M, Ioakeimidis F, Papadaki M, Kloukina I, Chrysanthou-Piterou M, Samiotaki M, Panayotou G, Matsas R, Douni E
PLoS genetics 2017 Apr;13(4):e1006656

---

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

---

CHCHD10 mutations promote loss of mitochondrial cristae junctions with impaired mitochondrial genome maintenance and inhibition of apoptosis.

Genin EC, Plutino M, Bannwarth S, Villa E, Cisneros-Barroso E, Roy M, Ortega-Vila B, Fragaki K, Lespinasse F, Pinero-Martos E, Augé G, Moore D, Burté F, Lacas-Gervais S, Kageyama Y, Itoh K, Yu-Wai-Man P, Sesaki H, Ricci JE, Vives-Bauza C, Paquis-Flucklinger V
EMBO molecular medicine 2016 Jan 1;8(1):58-72

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Lost region in amyloid precursor protein (APP) through TALEN-mediated genome editing alters mitochondrial morphology.

Wang Y, Wu F, Pan H, Zheng W, Feng C, Wang Y, Deng Z, Wang L, Luo J, Chen S
Scientific reports 2016 Feb 29;6:22244

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Mitochondrial hepato-encephalopathy due to deficiency of QIL1/MIC13 (C19orf70), a MICOS complex subunit.

Zeharia A, Friedman JR, Tobar A, Saada A, Konen O, Fellig Y, Shaag A, Nunnari J, Elpeleg O
European journal of human genetics : EJHG 2016 Dec;24(12):1778-1782

---

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.

Glytsou C, Calvo E, Cogliati S, Mehrotra A, Anastasia I, Rigoni G, Raimondi A, Shintani N, Loureiro M, Vazquez J, Pellegrini L, Enriquez JA, Scorrano L, Soriano ME
Cell reports 2016 Dec 13;17(11):3024-3034

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The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.

Petrungaro C, Zimmermann KM, Küttner V, Fischer M, Dengjel J, Bogeski I, Riemer J
Cell metabolism 2015 Oct 6;22(4):721-33

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Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Ding C, Wu Z, Huang L, Wang Y, Xue J, Chen S, Deng Z, Wang L, Song Z, Chen S
Scientific reports 2015 Nov 4;5:16064

---

Suppression of Drug Resistance in Dengue Virus.

Mateo R, Nagamine CM, Kirkegaard K
mBio 2015 Dec 15;6(6):e01960-15

---

Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.

Huang R, Zhao L, Chen H, Yin RH, Li CY, Zhan YQ, Zhang JH, Ge CH, Yu M, Yang XM
PloS one 2014;9(5):e96246

---

The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.

Anand R, Wai T, Baker MJ, Kladt N, Schauss AC, Rugarli E, Langer T
The Journal of cell biology 2014 Mar 17;204(6):919-29

---

A mitochondrial origin for frontotemporal dementia and amyotrophic lateral sclerosis through CHCHD10 involvement.

Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore DG, Verschueren A, Rouzier C, Le Ber I, Augé G, Cochaud C, Lespinasse F, N'Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V
Brain : a journal of neurology 2014 Aug;137(Pt 8):2329-45

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CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology.

An J, Shi J, He Q, Lui K, Liu Y, Huang Y, Sheikh MS
The Journal of biological chemistry 2012 Mar 2;287(10):7411-26

---

Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis.

Jiang L, Fang J, Moore DS, Gogichaeva NV, Galeva NA, Michaelis ML, Zaidi A
Neurobiology of aging 2010 Dec;31(12):2146-59

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Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking.

Weihofen A, Thomas KJ, Ostaszewski BL, Cookson MR, Selkoe DJ
Biochemistry 2009 Mar 10;48(9):2045-52

---

Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells.

Yin X, Mayr M, Xiao Q, Wang W, Xu Q
Proteomics 2006 Dec;6(24):6437-46

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The Mitofilin antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Mitofilin. This antibody recognizes human, mouse antigen. The Mitofilin antibody has been validated for the following applications: ELISA, IHC, IF, WB, IP analysis.

Appendix

10179-1-AP.pdf

Show more

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using IMMT antibody 10179-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-IMMT (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 10179-1-AP(IMMT antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 10179-1-AP(IMMT antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue