Explore
Validate
Learn
Western blot
ELISA
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C678336 - Provider product page
- Provider
- LSBio
- Product name
- IMMT / Mitofilin Antibody LS-C678336
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in:Hela whole cell lysate,HepG2 whole cell lysate,MCF-7 whole cell lysate All Lanes:IMMT antibody at 3.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84,83,81 KDa Observed band size: 84 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating IMMT in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in HepG2 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating IMMT in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in HepG2 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating IMMT in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in HepG2 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
