PA1-16918

antibody from Invitrogen Antibodies

Targeting: IMMT HMP, Mic60, MICOS60, MINOS2, P87, P89

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16918

Provider

Invitrogen Antibodies

Product name

Mitofilin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Suggested positive control: HeLa whole cell lysate, dog testis, antigen standard for IMMT (transient overexpression lysate).

Reactivity

Human, Mouse, Rat, Canine, Feline

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20°C or -80°C if preferred

References

Submitted references

Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA.

Federspiel JD, Cook KC, Kennedy MA, Venkatesh SS, Otter CJ, Hofstadter WA, Jean Beltran PM, Cristea IM
Cell reports 2020 Jul 28;32(4):107943

---

Mitochondrial p53 mediates a transcription-independent regulation of cell respiration and interacts with the mitochondrial F₁F0-ATP synthase.

Bergeaud M, Mathieu L, Guillaume A, Moll UM, Mignotte B, Le Floch N, Vayssière JL, Rincheval V
Cell cycle (Georgetown, Tex.) 2013 Sep 1;12(17):2781-93

---

Mitochondrial localization of PARP-1 requires interaction with mitofilin and is involved in the maintenance of mitochondrial DNA integrity.

Rossi MN, Carbone M, Mostocotto C, Mancone C, Tripodi M, Maione R, Amati P
The Journal of biological chemistry 2009 Nov 13;284(46):31616-24

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of dog testis total protein extract 1:2000 dilution of antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Mitofilin in dog testis. Samples were incubated in Mitofilin polyclonal antibody (Product # PA1-16918) using a dilution of 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of HeLa cells with antibody 1:500 dilution labeling mitochondria

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Mitofilin in HeLa cells. Samples were incubated in Mitofilin polyclonal antibody (Product # PA1-16918) using a dilution of 1:500. Antibody labeling mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Mitofilin in a section of normal human skin. Samples were incubated in Mitofilin polyclonal antibody (Product # PA1-16918) using a dilution of 1:200. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification].

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Mitofilin in a section of normal human skin. Samples were incubated in Mitofilin polyclonal antibody (Product # PA1-16918) using a dilution of 1:200. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification].

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Mitofilin in a transverse section of normal human skeletal muscle. Samples were incubated in Mitofilin polyclonal antibody (Product # PA1-16918) using a dilution of 1:200. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification].

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL