Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
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- Product number
- PA5-30419 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mitofilin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, MCF-7. Predicted reactivity: Mouse (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mitofilin Polyclonal Antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: Mitofilin protein stained by Mitofilin Polyclonal Antibody (Product # PA5-30419) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Mitofilin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Mitofilin Polyclonal Antibody (Product # PA5-30419) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: blue) were stained with Hoechst33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000). Panel d represents the merged image showing cytoplasmic (mitochondria-like) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mitofilin Polyclonal Antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: Mitofilin protein stained by Mitofilin Polyclonal Antibody (Product # PA5-30419) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Mitofilin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Mitofilin Polyclonal Antibody (Product # PA5-30419) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: blue) were stained with Hoechst33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000). Panel d represents the merged image showing cytoplasmic (mitochondria-like) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).