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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-56251 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Layilin Monoclonal Antibody (K94011_9G2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: KRKREQPDPS TKKQHTIWPS PHQGNSPDLE VYNVIRKQSE ADLAETRPDL KNISFRVCSG EATPDDMSCD YDNMAVNPSE SGFVTLVSVE SGFVTNDIYE FSPDQMGRSK
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K94011_9G2
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Layilin in various protein samples (Lane 1: 15 µg of MCF7 lysate, Lane 2: 15 µg of U-251MG lysate, Lane 3: 15 µg of K-562 lysate). Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, followed by peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Layilin band was visualized using ECL Substrate. Incubation with primary Layilin monoclonal antibody (Product # MA5-55871) at a dilution of 1 µg/mL was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Layilin in 200 µg of K-562 lysate. Samples are as follows: Lane 1: K-562 lysate, Lane 2: Layilin immunoprecipitated from K-562 lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with Layilin monoclonal antibody (Product # MA5-55871) at a dilution of 2.5 µg was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Layilin in paraffin-embedded stomach tissue. Sample was incubated with Layilin monoclonal antibody (Product # MA5-55871) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
