Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30613 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOSIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Raji, mouse brain. Predicted reactivity: Mouse (89%), Rat (90%), Rhesus Monkey (99%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NOSIP Polyclonal Antibody (Product # PA5-30613). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 12% SDS PAGE. NOSIP Polyclonal Antibody (Product # PA5-30613) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NOSIP Polyclonal Antibody (Product # PA5-30613). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. NOSIP Polyclonal Antibody (Product # PA5-30613) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NOSIP in paraformaldehyde-fixed A431 cells using a NOSIP polyclonal antibody (Product # PA5-30613) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NOSIP was performed in paraffin-embedded human colon carcinoma tissue using NOSIP Polyclonal Antibody (Product # PA5-30613) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NOSIP (Product # PA5-30613) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.