14025-1-AP

antibody from Proteintech Group

Targeting: SOCS3 CIS3, Cish3, SOCS-3, SSI-3

Provider product page for 14025-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [64]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14025-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14025-1-AP, RRID:AB_10597854
Product name: SOCS3 antibody
Antibody type: Polyclonal
Description: KD/KO validated SOCS3 antibody (Cat. #14025-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SOCS3 protein structure - 14025-1-AP shown in red.

Submitted references Myeloid Deletion of Cdc42 Protects Liver From Hepatic Ischemia-Reperfusion Injury via Inhibiting Macrophage-Mediated Inflammation in Mice.

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Transcriptional regulation of suppressors of cytokine signaling during infection with Mycobacterium tuberculosis in human THP-1-derived macrophages and in mice.

Roy T, Seth A, Shafi H, Reddy DVS, Raman SK, Chakradhar JVUS, Verma S, Bharti R, Azmi L, Ray L, Misra A
Microbes and infection 2024 Mar-Apr;26(3):105282

Iron overload in hypothalamic AgRP neurons contributes to obesity and related metabolic disorders.

Zhang Y, Chen L, Xuan Y, Zhang L, Tian W, Zhu Y, Wang J, Wang X, Qiu J, Yu J, Tang M, He Z, Zhang H, Chen S, Shen Y, Wang S, Zhang R, Xu L, Ma X, Liao Y, Hu C
Cell reports 2024 Mar 26;43(3):113900

Enhancing precision medicine: a nomogram for predicting platinum resistance in epithelial ovarian cancer.

Li R, Xiong Z, Ma Y, Li Y, Yang Y, Ma S, Ha C
World journal of surgical oncology 2024 Mar 21;22(1):81

5-AZA Upregulates SOCS3 and PTPN6/SHP1, Inhibiting STAT3 and Potentiating the Effects of AG490 against Primary Effusion Lymphoma Cells.

Di Crosta M, Arena A, Benedetti R, Gilardini Montani MS, Cirone M
Current issues in molecular biology 2024 Mar 14;46(3):2468-2479

MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer.

Gu X, Wei F, Tong J, Liu Y, Chen S, Zheng L, Xing Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan;38(1):e23388

NOTCH1 as a Negative Regulator of Avian Adipocyte Differentiation: Implications for Fat Deposition.

Wang Z, Su Y, Zhao M, Ma Z, Li J, Hou Z, Li H
Animals : an open access journal from MDPI 2024 Feb 9;14(4)

A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory target gene expression.

Gaggioli MR, Jones AG, Panagi I, Washington EJ, Loney RE, Muench JH, Brennan RG, Thurston TLM, Ko DC
bioRxiv : the preprint server for biology 2024 Feb 14;

Salmonella Pullorum effector SteE regulates Th1/Th2 cytokine expression by triggering the STAT3/SOCS3 pathway that suppresses NF-κB activation.

Liu Z, Wang L, Gao P, Yu Y, Zhang Y, Fotin A, Wang Q, Xu Z, Wei X, Fotina T, Ma J
Veterinary microbiology 2023 Sep;284:109817

TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes.

Ben-Khemis M, Liu D, Pintard C, Song Z, Hurtado-Nedelec M, Marie JC, El-Benna J, Dang PM
Redox biology 2023 Nov;67:102898

Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death.

Chen X, Chen J, Miao C, Yin G, Zhang Z, Sun R, Ni S
Molecular medicine reports 2023 Nov;28(5)

Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation.

Wu M, Song D, Li H, Ahmad N, Xu H, Yang X, Wang Q, Cheng X, Deng S, Shu X
International journal of molecular sciences 2023 May 29;24(11)

Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication.

Kao YS, Wang LC, Chang PC, Lin HM, Lin YS, Yu CY, Chen CC, Lin CF, Yeh TM, Wan SW, Wang JR, Ho TS, Chu CC, Zhang BC, Chang CP
PLoS pathogens 2023 Mar;19(3):e1011241

Endothelial progenitor cell-derived exosomes promote anti-inflammatory macrophages via SOCS3/JAK2/STAT3 axis and improve the outcome of spinal cord injury.

Yuan F, Peng W, Yang Y, Xu J, Liu Y, Xie Y, Huang T, Shi C, Ding Y, Li C, Qin T, Xie S, Zhu F, Lu H, Huang J, Hu J
Journal of neuroinflammation 2023 Jun 30;20(1):156

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner.

Wu G, Su Q, Li J, Xue C, Zhu J, Cai Q, Huang J, Ji S, Cheng B, Ge H
Journal of nanobiotechnology 2023 Jan 5;21(1):7

Involvement of METTL3 in arsenite-induced skin lesions by targeting the SOCS3/STAT3/Krt signaling pathway.

Yang F, Zhang A
Environmental pollution (Barking, Essex : 1987) 2023 Jan 1;316(Pt 2):120634

Interferon gamma regulates a complex pro-survival signal network in chronic lymphocytic leukemia.

Chen Y, Shao X, Yang H, Ren L, Cui Y, Zhang W, Macip S, Meng X
European journal of haematology 2023 Apr;110(4):435-443

Effect of Platycodon grandiflorus Polysaccharide on M1 Polarization Induced by Autophagy Degradation of SOCS1/2 Proteins in 3D4/21 Cells.

Li L, Chen X, Lv M, Cheng Z, Liu F, Wang Y, Zhou A, Liu J, Zhao X
Frontiers in immunology 2022;13:934084

YTHDF1 Negatively Regulates Treponema pallidum-Induced Inflammation in THP-1 Macrophages by Promoting SOCS3 Translation in an m6A-Dependent Manner.

Li Z, Teng M, Jiang Y, Zhang L, Luo X, Liao Y, Yang B
Frontiers in immunology 2022;13:857727

Blocking STAT3 signaling augments MEK/ERK inhibitor efficacy in esophageal squamous cell carcinoma.

Zheng ZY, Chu MY, Lin W, Zheng YQ, Xu XE, Chen Y, Liao LD, Wu ZY, Wang SH, Li EM, Xu LY
Cell death & disease 2022 May 25;13(5):496

Effect of 1-Deoxynojirimycin on insulin resistance in prediabetic mice based on next-generation sequencing and intestinal microbiota study.

Ren X, Xing Y, He L, Xiu Z, Yang L, Han A, Jia Q, Dong Y
Journal of ethnopharmacology 2022 May 10;289:115029

Inhibition of Transient Receptor Potential Vanilloid 4 (TRPV4) Mitigates Seizures.

Zeng ML, Cheng JJ, Kong S, Yang XL, Jia XL, Cheng XL, Chen L, He FG, Liu YM, Fan YT, Gongga L, Chen TX, Liu WH, He XH, Peng BW
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2022 Mar;19(2):660-681

Chronic Central Leptin Infusion Promotes an Anti-Inflammatory Cytokine Profile Related to the Activation of Insulin Signaling in the Gastrocnemius of Male Rats.

Barrios V, Guerra-Cantera S, Martín-Rivada Á, Canelles S, Campillo-Calatayud A, Arilla-Ferreiro E, Frago LM, Chowen JA, Argente J
Biomedicines 2022 Jun 21;10(7)

Neuroglobin alleviates the neurotoxicity of sevoflurane to fetal rats by inhibiting neuroinflammation and affecting microglial polarization.

Zhang Y, Gao Y, Yang F, Wu X, Tang Z, Liu H
Brain research bulletin 2022 Jun 1;183:142-152

GPR109a Regulates Phenotypic and Functional Alterations in Macrophages and the Progression of Type 1 Diabetes.

Zhang Z, Li J, Zhang M, Li B, Pan X, Dong X, Pan LL, Sun J
Molecular nutrition & food research 2022 Dec;66(23):e2200300

Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network.

Huang ZH, Wang H, Wang DM, Zhao XY, Liu WW, Zhong X, He DM, Mu BR, Lu MH
Journal of cellular and molecular medicine 2022 Dec;26(23):5779-5793

Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.

Tian D, Teng X, Jin S, Chen Y, Xue H, Xiao L, Wu Y
Journal of advanced research 2021 Jan;27:115-125

Pro-protein convertase subtilisin/kexin type 9 promotes intestinal tumor development by activating Janus kinase 2/signal transducer and activator of transcription 3/SOCS3 signaling in Apc(Min/+) mice.

Yang K, Zhu J, Luo HH, Yu SW, Wang L
International journal of immunopathology and pharmacology 2021 Jan-Dec;35:20587384211038345

GCSF deficiency attenuates nonalcoholic fatty liver disease through regulating GCSFR-SOCS3-JAK-STAT3 pathway and immune cells infiltration.

Zhang Y, Zhou X, Liu P, Chen X, Zhang J, Zhang H, Li S, Chen Y, Song X, Wang J, Zeng H, Zhang X, Tang C, Yu C, Li Y, Xu C
American journal of physiology. Gastrointestinal and liver physiology 2021 Apr 1;320(4):G531-G542

Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells.

Yuan C, Yang D, Ma J, Yang J, Xue J, Song F, Liu X
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2020;53(8):e9488

Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation.

Jiang L, Chen T, Xiong L, Xu JH, Gong AY, Dai B, Wu G, Zhu K, Lu E, Mathy NW, Chen XM
Oncology letters 2020 Sep;20(3):2191-2198

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Ebokaiwe AP, Njoya EM, Sheng Y, Zhang Z, Li S, Zhou Z, Qiang Z, Peng T, Hussein AA, Zhang G, Lu X, Li L, Wang F
Toxicology and applied pharmacology 2020 Oct 1;404:115203

Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Cai BB, Lu YN, Xu M
Acta pharmacologica Sinica 2020 May;41(5):650-660

gga-miR-27b-3p enhances type I interferon expression and suppresses infectious bursal disease virus replication via targeting cellular suppressors of cytokine signaling 3 and 6 (SOCS3 and 6).

Duan X, Zhao M, Li X, Gao L, Cao H, Wang Y, Zheng SJ
Virus research 2020 May;281:197910

Long non-coding RNA expression profiling following treatment with resveratrol to improve insulin resistance.

Shu L, Hou G, Zhao H, Huang W, Song G, Ma H
Molecular medicine reports 2020 Aug;22(2):1303-1316

Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.

Shiau DJ, Kuo WT, Davuluri GVN, Shieh CC, Tsai PJ, Chen CC, Lin YS, Wu YZ, Hsiao YP, Chang CP
Scientific reports 2020 Aug 12;10(1):13582

Expression and methylation levels of suppressor of cytokine signaling 3 in rheumatic arthritis synovial fibroblasts.

Meng C, Qi H, Wang X, Wu X, Huang W, Wang H, He Y
Experimental and molecular pathology 2020 Apr;113:104361

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Zhu C, Zou C, Guan G, Guo Q, Yan Z, Liu T, Shen S, Xu X, Chen C, Lin Z, Cheng W, Wu A
Oncoimmunology 2019;8(9):e1621677

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Wang D, Xu B, Wang J, Wang H, Guo J, Ji H, Li S, Wu R, Yang H, Lian S
Brain research 2019 Nov 1;1722:146354

Adeno-associated virus 9-mediated RNA interference targeting SOCS3 alleviates diastolic heart failure in rats.

Gao J, Guo Y, Chen Y, Zhou J, Liu Y, Su P
Gene 2019 May 20;697:11-18

Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection.

Wang J, Teng Y, Zhao G, Li F, Hou A, Sun B, Kong W, Gao F, Cai L, Jiang C
International journal of molecular sciences 2019 Mar 27;20(7)

Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1.

Zhao G, Gong L, Su D, Jin Y, Guo C, Yue M, Yao S, Qin Z, Ye Y, Tang Y, Wu Q, Zhang J, Cui B, Ding Q, Huang H, Hu L, Chen Y, Zhang P, Hu G, Chen L, Wong KK, Gao D, Ji H
The Journal of clinical investigation 2019 Mar 1;129(3):972-987

Low doses of decitabine improve the chemotherapy efficacy against basal-like bladder cancer by targeting cancer stem cells.

Wu M, Sheng L, Cheng M, Zhang H, Jiang Y, Lin S, Liang Y, Zhu F, Liu Z, Zhang Y, Zhang X, Gao Q, Chen D, Li J, Li Y
Oncogene 2019 Jul;38(27):5425-5439

High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.

Wang J, Gao WJ, Deng SL, Liu X, Jia H, Ma WZ
Stem cell research & therapy 2019 Jul 29;10(1):227

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.

Zhu Y, Meng Y, Zhao Y, Zhu J, Xu H, Zhang E, Shi L, Du L, Liu G, Zhang C, Xu X, Kang X, Zhen Y, Zhang S
Colloids and surfaces. B, Biointerfaces 2019 Jul 1;179:66-76

m(6)A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Wu R, Liu Y, Zhao Y, Bi Z, Yao Y, Liu Q, Wang F, Wang Y, Wang X
Cell death & disease 2019 Feb 20;10(3):171

Neonatal Overnutrition Increases Testicular Size and Expression of Luteinizing Hormone β-Subunit in Peripubertal Male Rats.

Argente-Arizón P, Castro-González D, Díaz F, Fernández-Gómez MJ, Sánchez-Garrido MA, Tena-Sempere M, Argente J, Chowen JA
Frontiers in endocrinology 2018;9:168

MBD2-mediated Th17 differentiation in severe asthma is associated with impaired SOCS3 expression.

Sun W, Xiao B, Jia A, Qiu L, Zeng Q, Liu D, Yuan Y, Jia J, Zhang X, Xiang X
Experimental cell research 2018 Oct 1;371(1):196-204

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Lian S, Wang D, Xu B, Guo W, Wang L, Li W, Ji H, Wang J, Kong F, Zhen L, Li S, Zhang L, Guo J, Yang H
Behavioural brain research 2018 Jul 2;346:1-10

The Hypothalamic Inflammatory/Gliosis Response to Neonatal Overnutrition Is Sex and Age Dependent.

Argente-Arizón P, Díaz F, Ros P, Barrios V, Tena-Sempere M, García-Segura LM, Argente J, Chowen JA
Endocrinology 2018 Jan 1;159(1):368-387

Di-(2-ethylhexyl)-phthalate induces glucose metabolic disorder in adolescent rats.

Xu J, Zhou L, Wang S, Zhu J, Liu T, Jia Y, Sun D, Chen H, Wang Q, Xu F, Zhang Y, Liu H, Zhang T, Ye L
Environmental science and pollution research international 2018 Feb;25(4):3596-3607

Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor-dependent signaling in human gingival epithelial cells.

Elkaim R, Bugueno-Valdebenito IM, Benkirane-Jessel N, Tenenbaum H
Journal of oral microbiology 2017;9(1):1334503

Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.

Fang L, Hodge J, Saaoud F, Wang J, Iwanowycz S, Wang Y, Hui Y, Evans TD, Razani B, Fan D
Oncoimmunology 2017;6(5):e1312042

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Igbe I, Shen XF, Jiao W, Qiang Z, Deng T, Li S, Liu WL, Liu HW, Zhang GL, Wang F
Oncotarget 2017 Dec 26;8(69):113734-113748

6-Hydroxy-3-O-methyl-kaempferol 6-O-glucopyranoside potentiates the anti-proliferative effect of interferon α/β by promoting activation of the JAK/STAT signaling by inhibiting SOCS3 in hepatocellular carcinoma cells.

Wonganan O, He YJ, Shen XF, Wongkrajang K, Suksamrarn A, Zhang GL, Wang F
Toxicology and applied pharmacology 2017 Dec 1;336:31-39

MiR-155 Enhances Insulin Sensitivity by Coordinated Regulation of Multiple Genes in Mice.

Lin X, Qin Y, Jia J, Lin T, Lin X, Chen L, Zeng H, Han Y, Wu L, Huang S, Wang M, Huang S, Xie R, Liang L, Liu Y, Liu R, Zhang T, Li J, Wang S, Sun P, Huang W, Yao K, Xu K, Du T, Xiao D
PLoS genetics 2016 Oct;12(10):e1006308

Suppressors of cytokine signaling 3 is essential for FcγR-mediated inflammatory response via enhancing CCAAT/enhancer-binding protein δ transcriptional activity in macrophages.

Yan C, Liu Y, Gao H, Wang X
Experimental cell research 2015 Sep 10;337(1):120-7

IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

Dong Z, Lu X, Yang Y, Zhang T, Li Y, Chai Y, Lei W, Li C, Ai L, Tai W
BMC pulmonary medicine 2015 Feb 18;15:13

Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.

Susnik N, Sörensen-Zender I, Rong S, von Vietinghoff S, Lu X, Rubera I, Tauc M, Falk CS, Alexander WS, Melk A, Haller H, Schmitt R
Kidney international 2014 Jun;85(6):1357-68

Kallikrein-binding protein inhibits LPS-induced TNF-α by upregulating SOCS3 expression.

Dai Z, Lu L, Yang Z, Mao Y, Lu J, Li C, Qi W, Chen Y, Yao Y, Li L, Chen S, Zhang Y, Cai W, Yang X, Gao G
Journal of cellular biochemistry 2013 May;114(5):1020-8

TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.

Chang CP, Su YC, Hu CW, Lei HY
Cell death and differentiation 2013 Mar;20(3):515-23

Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cDNA library.

Luo B, Zou T, Lu N, Chai F, Ye X, Wang Y, Qi Y
Biochemical and biophysical research communications 2011 Dec 9;416(1-2):39-44

The role of microtubule-associated protein 1S in SOCS3 regulation of IL-6 signaling.

Zou T, Ouyang L, Chen L, Dong W, Qiao H, Liu Y, Qi Y
FEBS letters 2008 Dec 10;582(29):4015-22

BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.

Liu Y, Dong W, Chen L, Xiang R, Xiao H, De G, Wang Z, Qi Y
The Journal of biological chemistry 2004 Sep 3;279(36):37436-44

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 14025-1-AP(SOCS3 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using SOCS3 antibody 14025-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human placenta using 14025-1-AP(SOCS3 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human placenta using 14025-1-AP(SOCS3 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue