Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502922 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502922, RRID:AB_11126046
- Product name
- SOCS3 mouse monoclonal antibody, clone OTI6F3 (formerly 6F3)
- Antibody type
- Monoclonal
- Description
- SOCS3 mouse monoclonal antibody, clone OTI6F3 (formerly 6F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOCS3
- Isotype
- IgG
- Antibody clone number
- OTI6F3
- Vial size
- 100 µl
- Concentration
- 0.76 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209305 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOCS3 antibody(TA502922), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-SOCS3 antibody(TA502922),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC