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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- TA502991 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502991, RRID:AB_11126495
- Product name
- SOCS3 mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)
- Antibody type
- Monoclonal
- Description
- SOCS3 mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOCS3
- Isotype
- IgG
- Antibody clone number
- OTI3D3
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation.
Lin CR, Wei TY, Tsai HY, Wu YT, Wu PY, Chen ST
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Dec;29(12):5006-17
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Dec;29(12):5006-17
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SOCS3 mouse monoclonal antibody (TA502991) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502991)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502991)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SOCS3 antibody(TA502991),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-SOCS3 antibody(TA502991),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
