TA503025

antibody from OriGene

Targeting: SOCS3 CIS3, Cish3, SOCS-3, SSI-3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503025

Provider

OriGene

Proper citation

OriGene Cat#TA503025, RRID:AB_11125289

Product name

SOCS3 mouse monoclonal antibody, clone OTI6C2 (formerly 6C2)

Antibody type

Monoclonal

Description

SOCS3 mouse monoclonal antibody, clone OTI6C2 (formerly 6C2)

Host

Mouse

Conjugate

Unconjugated

Epitope

SOCS3

Isotype

IgG

Antibody clone number

OTI6C2

Vial size

100 µl

Concentration

1.2 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SOCS3 mouse monoclonal antibody (TA503025) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209305 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOCS3 antibody(TA503025), and then analyzed by flow cytometry.

Validation comment

FC