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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-45435 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Slo2.2 Monoclonal Antibody (N3/26), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-45435 was sufficient for detection of Slo2.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa. Weak human detection.approximately Does not cross-react with KCNT2/Slo2.1/Slick.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- N3/26
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein.2 Potassium Channel. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with Slo2.2 Potassium Channel monoclonal antibody (Product # MA5-45435) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol for 10 minutes at -20°C. Samples were incubated with Slo2.2 Potassium Channel monoclonal antibody (Product # MA5-45435) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Slo2.2 monoclonal antibody (Product # MA5-45435) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Slo2.2 antibody (D) Composite.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using mouse backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Slo2.2 Potassium Channel monoclonal antibody (Product # MA5-45435) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.
- Conjugate
- Green dye
