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Western blot
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Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [5]
- Immunohistochemistry [9]
- Other assay [1]
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- Product number
- 17711-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARL13B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
ARL13B protein structure - 17711-1-AP shown in red.
Antigen information not provided.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG (H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 66200-1-Ig.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP ( ARL13B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP ( ARL13B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.
