TA324534

antibody from OriGene

Targeting: NANOS1 NOS1, ZC2HC12A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324534

Provider

OriGene

Product name

Rabbit polyclonal Nanos Homologue 1 (NANOS1) Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal Nanos Homologue 1 (NANOS1) Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

NANOS1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) (Cat.#TA324534), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

FC